Lipids

, Volume 20, Issue 1, pp 29–39

A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

  • Graham W. Burton
  • Ann Webb
  • Keith U. Ingold
Methods

DOI: 10.1007/BF02534359

Cite this article as:
Burton, G.W., Webb, A. & Ingold, K.U. Lipids (1985) 20: 29. doi:10.1007/BF02534359

Abstract

A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol andn-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol.

Abbreviations

SDS

sodium dodecyl sulfate

MLV

multilamellar vesicles

RBC

red blood cells

PC

phosphatidylcholine

PE

phosphatidylethanolamine

PS

phosphatidylserine

SP

sphingomyelin

2-BHA

2-t-butyl-4-hydroxyanisole

PMHC

2,2,5,7,8-pentamethyl-6-hydroxychroman

CMC

critical micelle concentration

Copyright information

© American Oil Chemists’ Society (AOCS) 1985

Authors and Affiliations

  • Graham W. Burton
    • 1
  • Ann Webb
    • 1
  • Keith U. Ingold
    • 1
  1. 1.Division of ChemistryNational Research Council of CanadaOttawaCanada

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