Lipids

, Volume 15, Issue 9, pp 609–615

Biosynthesis of hydrocarbons by algae: Decarboxylation of stearic acid to N-heptadecane inAnacystis nidulans determined by13C- and2H-labeling and13C nuclear magnetic resonance

  • A. Gavin McInnes
  • John A. Walter
  • Jeffrey L. C. Wright
Article

DOI: 10.1007/BF02534009

Cite this article as:
McInnes, A.G., Walter, J.A. & Wright, J.L.C. Lipids (1980) 15: 609. doi:10.1007/BF02534009

Abstract

The distribution of isotopic labels inn-heptadecane enriched from [1,2-13C] and [2-13C, 2-2H3) acetates byAnacystis nidulans has been determined by13C nuclear magnetic resonance (13C NMR). Labeling with [1,2-13C] acetate is consistent with assembly from13C−13C units derived from an acetate “starter” group and 8 malonate units, as in fatty acid biosynthesis, followed by production of a methyl group through bond cleavage of the terminal13C−13C unit. A comparison of the hydrocarbon with palmitic acid (the only fatty acid produced in sufficient amount for NMR analysis) enriched from [2-13C,2-2H3]acetate by the same culture shows that they have retained the same fraction of2H at corresponding sites, and have therefore undergone identical biosynthetic and hydrogen-deuterium exchange processes, as would be expected ifn-heptadecane originates from de novo-synthesized stearic acid.

Copyright information

© American Oil Chemists’ Society 1980

Authors and Affiliations

  • A. Gavin McInnes
    • 1
  • John A. Walter
    • 1
  • Jeffrey L. C. Wright
    • 1
  1. 1.Atlantic Research LaboratoryNational Research Council of CanadaHalifaxCanada