Lipids

, Volume 12, Issue 8, pp 645–654

Sterol mutants ofSaccharomyces cerevisiae: Chromatographic analyses

  • M. Bard
  • R. A. Woods
  • D. H. R. Barton
  • J. E. T. Corrie
  • D. A. Widdowson
Article

DOI: 10.1007/BF02533759

Cite this article as:
Bard, M., Woods, R.A., Barton, D.H.R. et al. Lipids (1977) 12: 645. doi:10.1007/BF02533759

Abstract

The sterols accumulated by ergosterol deficient mutants of the geneserg6, erg2, erg3, anderg5 (formerlypo11, po12, po13, andpo15) have been analyzed by gas liquid chromatography. Together with pure sterols obtained from the mutants, they were characterized on SE-30, OV-17, and OV-225. The effects of molecular structure on the retention characteristics of a range of C28 ergostane sterols have been studied. The double mutants obtained by crossing the single mutants were also analyzed and their sterols identified where possible. The effects of theerg mutations on the control of sterol biosynthesis in yeast are discussed.

Copyright information

© American Oil Chemists’ Society 1977

Authors and Affiliations

  • M. Bard
    • 1
  • R. A. Woods
    • 1
  • D. H. R. Barton
    • 2
  • J. E. T. Corrie
    • 2
  • D. A. Widdowson
    • 2
  1. 1.Department of GeneticsUniversity of SheffieldSheffieldEngland
  2. 2.Department of ChemistryImperial CollegeLondonEngland
  3. 3.Department of BiologyIndiana University, Purdue University at IndianapolisIndianapolis
  4. 4.Department of BiologyUniversity of WinnipegWinnipegCanada