Lipids

, Volume 13, Issue 7, pp 497–503

Generation of phospholipid artefacts during extraction of developing soybean seeds with methanolic solvents

  • P. G. Roughan
  • C. R. Slack
  • R. Holland
Article

DOI: 10.1007/BF02533620

Cite this article as:
Roughan, P.G., Slack, C.R. & Holland, R. Lipids (1978) 13: 497. doi:10.1007/BF02533620

Abstract

The major phospholipids of soybean cotyledons during development were phosphatidylcholine (45–55%), phosphatidylethanolamine (24–28%), and phosphatidylinositol (15–18%) when the tissue was steam-killed prior to extraction of the lipids. The only other phospholipids of any significance (4–6%) was identified as phosphatidylglycerol. Phosphatidic acid was a minor constituent (<1%), and neither N-acyl phosphatidylethanolamine norbis-phosphatidic acid were detected in appreciable (>0.1% of the total lipid phosphorus) quantities. When fresh cotyledons were rapidly homogenized in mixtures of chloroform and methanol or in methanol alone, phosphatidylmethanol was formed in variable amounts (0–20% of the total phospholipid), and when cotyledons were soaked in methanol prior to homogenizing, phosphatidylmethanol became the major phospholipid, accounting for up to 75% of the total lipid phosphorus. Phosphatidylmethanol was formed by the phospholipase D-catalyzed transphosphatidylation of phosphatidylcholine and phosphatidylethanolamine during extraction.

Copyright information

© American Oil Chemists’ Society 1978

Authors and Affiliations

  • P. G. Roughan
    • 1
  • C. R. Slack
    • 1
  • R. Holland
    • 1
  1. 1.Plant Physiology DivisionD.S.I.R.Palmerston NorthNew Zealand