Archives of Microbiology

, Volume 164, Issue 5, pp 331–336

Thermodesulforhabdus norvegicus gen. nov., sp. nov., a novel thermophilic sulfate-reducing bacterium from oil field water

Original Paper

DOI: 10.1007/BF02529979

Cite this article as:
Beeder, J., Torsvik, T. & Lien, T. Arch. Microbiol. (1995) 164: 331. doi:10.1007/BF02529979

Abstract

A novel gram-negative, thermophilic, acetate-oxidizing, sulfate-reducing bacterium, strain A8444, isolated from hot North Sea oil field water, is described. The rod-shaped cells averaged 1 μm in width and 2.5 μm in length. They were motile by means of a single polar flagellum. Growth was observed between 44 and 74°C, with an optimum at 60°C. Spores were not produced. Sulfate and sulfite were used as electron acceptors. Sulfur, thiosulfate, nitrate, fumarate, and pyruvate were not reduced. In the presence of sulfate, growth was observed with acetate, lactate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, hepatanoate, octanoate, nonadecanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate, stearate, and ethanol. Pyruvate, lactate, and fumarate did not support fermentative growth. Cytochromes of thec-type were present. Desulfoviridin, desulforubidin, P582, and desulfofuscidin were not present. The G+C content of the DNA was 51 mol%. Sequence analysis of 16S rDNA showed that phylogenetically strain A8444 belongs to the delta subdivision of the Proteobacteria. The closest relatives areDesulfacinum infernum andSyntrophobacter wolinii. Strain A8444 is described as the type strain of the new taxonThermodesulforhabdus norvegicus gen. nov., sp. nov.

Key words

Sulfate reductionThermophileOil field waterAcetate oxidationDelta subdivisionThermodesulforhabdus norvegicus gen. nov., sp. nov

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of BergenBergenNorway