Calcified Tissue International

, Volume 58, Issue 2, pp 101–108

Bone marrow stromal cells are load responsivein vitro

  • G. P. Thomas
  • A. J. El Haj
Laboratory Investigations

DOI: 10.1007/BF02529731

Cite this article as:
Thomas, G.P. & El Haj, A.J. Calcif Tissue Int (1996) 58: 101. doi:10.1007/BF02529731


Mechanical load-related effects on bone marrow stromal cellsin vitro have been investigated. A dose response of a cyclical load of 1 Hz between 350 ustrain and 2500 ustrain applied to 10-day-old cultures resulted in elevated alkaline phosphatase levels and the number of cells expressing this protein after 2 days. No significant changes in the number of cells expressing or the production of collagen type I was observed. A critical stage of development of the cultures must be reached before load-related elevation in alkaline phosphatase expression could be measured independent of the stage at which loading was applied. Using a prostaglandin inhibitor at concentrations previously usedin vivo, the load response was abolished. We have demonstrated that bone marrow stromal cells are load responsive in culture and have made preliminary studies into determining the involvement of prostaglandins in this process.

Key words

Bone marrowMechanical loadDifferentiationProstaglandinsIn vitroBone cellsAlkaline phosphatase

Copyright information

© Springer-Verlag New York Inc 1996

Authors and Affiliations

  • G. P. Thomas
    • 1
  • A. J. El Haj
    • 1
  1. 1.School of Biological SciencesUniversity of BirminghamBirminghamUK