Transgenic Research

, Volume 1, Issue 6, pp 285–297

Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants

  • Jonathan D. G. Jones
  • L. Shlumukov
  • F. Carland
  • J. English
  • S. R. Scofield
  • G. J. Bishop
  • K. Harrison
Technical Note

DOI: 10.1007/BF02525170

Cite this article as:
Jones, J.D.G., Shlumukov, L., Carland, F. et al. Transgenic Research (1992) 1: 285. doi:10.1007/BF02525170

Abstract

Progress in plant molecular biology has depended heavily on the availability of effective vectors for plant cell transformation and heterologous expression. In this paper we describe the structures of a wide array of plasmids which have proved extremely effective in (a) plant transformation, (b) expression of heterologous genes and (c) assaying the activity of transposons in transgenic plants. Constructs that confer resistance to kanamycin, hygromycin, streptomycin, spectinomycin and phosphinotricin, or that confer β-glucuronidase (GUS) gene expression are presented. Binary vector constructs that carry polylinkers of the pUC and Bluescript types are also described. Plasmids that permit the expression of any heterologous reading frame from either nopaline synthase (nos) or octopine synthase (ocs) promoters, as well as the cauliflower mosaic virus 35S promoter, using either the nopaline synthase or octopine synthase 3′ polyadenylation sequences, are presented. These constructs permit a choice of orientation of the resulting transgene of interest, relative to the orientation of the selection marker gene. Most of the plasmids described here are publicly available.

Keywords

binary vectorplant expression plasmidkanamycin resistancespectinomycin resistancehygromycin resistancephosphinotricin resistancestreptomycin resistanceβ-glucuronidase

Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • Jonathan D. G. Jones
    • 1
    • 4
  • L. Shlumukov
    • 1
    • 3
    • 4
  • F. Carland
    • 2
    • 4
  • J. English
    • 1
  • S. R. Scofield
    • 1
  • G. J. Bishop
    • 1
  • K. Harrison
    • 1
  1. 1.The Sainsbury Laboratory, John Innes Centre for Plant Science ResearchNorwich Research ParkNorwichUK
  2. 2.Department of Plant PathologyUniversity of CaliforniaBerkeleyUSA
  3. 3.Institute of Cell Biology and Genetic EngineeringUkrainian Academy of SciencesKievUkraine
  4. 4.Advanced Genetic Sciences (now DNA Plant Technology Corporation)OaklandUSA
  5. 5.Department of BotanyUniversity of NottinghamNottinghamUK