Langerhans cell hyperplasia and IgE expression in canine atopic dermatitis

  • Thierry Olivry
  • Peter F. Moore
  • Verena K. Affolter
  • Diane K. Naydan
Original Paper

DOI: 10.1007/BF02505260

Cite this article as:
Olivry, T., Moore, P.F., Affolter, V.K. et al. Arch Dermatol Res (1996) 288: 579. doi:10.1007/BF02505260

Abstract

Langerhans cells appear to be critical for IgE-mediated allergen capture and presentation in human atopic dermatitis. The present study sought to determine whether epidermal (i.e Langerhans cells) and dermal dendritic cells in the skin of dogs with atopic dermatitis are hyperplastic and expressed surface IgE. Frozen sections of lesional or nonlesional atopic and normal control canine skin were immunostained with CD1a-, CD1c-, and IgE-specific monoclonal antibodies. The enumeration of cells was performed by morphometry in both the epidermis and the dermis. Cell counts were compared with each individual’s total serum IgE levels. Higher numbers of epidermal and dermal dendritic cells were present in atopic dogs than in normal control animals. Epidermal Langerhans cell counts were significantly higher in lesional than in nonlesional atopic specimens. IgE+ dendritic cells were observed in lesional atopic epidermis and dermis, and nonlesional atopic dermis, but not in normal control skin specimens. The percentages of IgE+ dendritic cells were correlated with each patient’s total serum IgE levels. These results demonstrate dendritic cell hyperplasia and IgE expression in canine atopic dermatitis. Increased epidermal Langerhans cell counts in lesional specimens suggest an epidermal allergen contact in canine atopic dermatitis.

Key words

DogsAtopic DermatitisLangerhans cellsDendritic cellsAntigen-presenting cellsImmunoglobulin-E

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Thierry Olivry
    • 1
  • Peter F. Moore
    • 1
  • Verena K. Affolter
    • 1
  • Diane K. Naydan
    • 1
  1. 1.Department of Pathology, Microbiology and Immunology, School of Veterinary MedicineUniversity of California DavisUSA
  2. 2.Department of Companion Animal & Special Species Medicine, College of Veterinary MedicineNorth Carolina State UniversityRaeighUSA