, Volume 54, Issue 9-10, pp 581-587

HPLC determination of chlorobenzenes, benzenesulphonyl chlorides and benzenesulphonic acids in industrial waste water

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An HPLC method has been developed for the determination of chlorobenzenes, benzenesulphonyl chlorides, benzenesulphonic acids and related compounds in industrial waste water. The separation and determination of diphenylsulphone, 1,4-dichlorobenzene, 1,2,3,4-tetrachlorobenzene 1,2,3,5-tetrachlorobenzene and pentachlorobenzene was performed in reversed-phase system with octadecylsilica gel stationary phase and aqueous-methanolic mobile phase. Successful separation of ionic and polar compounds as water pollutants such as: benzenesulphonamide, benzenesulphonic acid, 1,3-benzenedisulphonic acid, 4-chlorobenzenesulphonic acid, 2,5-dichlorobenzenesulphonyl chloride, 2,3,4-trichlorobenzenesulphonyl chloride and 2,4,5-trichlorobenzenesulphonyl chloride requires addition of a salt (sodium sulphate) to the mobile phase. Addition of a buffer or of an ion-pair reagent to aqueous-methanolic mobile phases did not yield satifactory improvement in separation selectivity nor of peak shape. Polar and ionic compounds diphenylsulphone and chlorobenzenes, were separated and determined by a two-step, reversed-phase LC method. On-line sample enrichment on an adsorption column packed either with octadecyl silica or with aminopropyl silica connected to an LC column improved the limits of detection of the pollutants.