Originals Column Liquid Chromatography

Chromatographia

, Volume 53, Supplement 1, pp S231-S235

First online:

Optimization of conditions for the liquid chromatographic-electrospray lonization-mass spectrometric analysis of amnesic shellfish poisoning toxins

  • N. PiñeiroAffiliated withDepartamento de Química Analítica y Alimentaria, Universidad de Vigo
  • , E. VaqueroAffiliated withDepartamento de Química Analítica y Alimentaria, Universidad de Vigo
  • , J. M. LeãoAffiliated withDepartamento de Química Analítica y Alimentaria, Universidad de Vigo
  • , A. Gogo-MartínezAffiliated withDepartamento de Química Analítica y Alimentaria, Universidad de Vigo
  • , J. A. Rodríguez VázquezAffiliated withDepartamento de Química Analítica y Alimentaria, Universidad de Vigo

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Summary

In this paper we report results obtained from analysis of domoic acid and its analogues, toxins responsible for incidents of amnesic shellfish poisoning (ASP) in several places world-wide, by use of liquid chromatography coupled with electrospray ionization mass spectrometry. Different chromatographic and mass spectrometry conditions were evaluated with the aim of achieving the best chromatographic performance combined with the optimum mass spectrometric response. From the results obtained it can be concluded that LC-MS is powerful tool for the selective and sensitive determination of domoic acid and its isomers in naturally contaminated samples.

Key Words

Liquid chromatography-mass spectrometry Amnesic shellfish poisoning toxins Optimization of parameters