The botanical magazine = Shokubutsu-gaku-zasshi

, Volume 85, Issue 1, pp 71–83

Occurrence of laccase and tyrosinase in fungi of agaricales and comparative study of laccase fromRussula delica andRussula pseudodelica

  • Teruo Matsubara
  • Hidekazu Iwasaki

DOI: 10.1007/BF02489202

Cite this article as:
Matsubara, T. & Iwasaki, H. Bot Mag Tokyo (1972) 85: 71. doi:10.1007/BF02489202


The activities of laccase and tyrosinase, that is, oxidation activities ofp-phenylenediamine andp-cresol in the fruiting bodies of species belonging to various genera of Agaricales were examined. Among 40 genera examined, appreciable laccase activities were observed in fungi belonging toLepista, Omphalina, Agaricus, Russula andLactarius. High typosinase activities were found in many species ofHygrophorus, Laccaria, Lepista, Melanoleuca, Collybia, Marasmius, Amanita, Agaricus, Inocybe, Pholiota, Russula andLactarius. Species of other genera exmained did not show either enzyme activities to any extent.

FromRussula delica andRussula pseudodelica, laccases were extracted and purified, and some characteristics were compared. Molecular weight and absorption spectra of these laccases were very similar to each other, but they were different in electrophoretical mobilities and substrate specificity.

Copyright information

© The Botanical Society of Japan 1972

Authors and Affiliations

  • Teruo Matsubara
    • 1
  • Hidekazu Iwasaki
    • 1
  1. 1.Biological Institute, Faculty of ScienceNagoya UniversityNagoya