Original Paper

Histochemistry and Cell Biology

, Volume 106, Issue 2, pp 247-251

Expression of the mouse testicular histone gene H1t during spermatogenesis

  • B. DrabentAffiliated withZentrum Biochemie, Abteilung Molekularbiologie, Universität Göttingen
  • , C. BodeAffiliated withZentrum Biochemie, Abteilung Molekularbiologie, Universität Göttingen
  • , B. BramlageAffiliated withZentrum Biochemie, Abteilung Molekularbiologie, Universität Göttingen
  • , D. DoeneckeAffiliated withZentrum Biochemie, Abteilung Molekularbiologie, Universität Göttingen

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Abstract

The testicular H1 histone variant, H1t, is synthesized during spermatogenesis in mammalian male germ cells. In situ hybridization and immunohistochemical techniques were used to assign the expression of either the H1t mRNA or the H1t protein to specific cell stages of spermatogenesis. Our results show the presence of the H1t mRNA only in the late and mid-pachytene stages, whereas the protein occurs first in pachytene spermatocytes, and persists until later stages from round up to elongated spermatids.