Oxygen-induced retinopathy in the rat: Possible contribution of peroxidation reactions
- John S. Penn
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Albino rats were maintained in 60% atmospheric oxygen from birth through 14 days of age. Age-matched controls were simultaneously raised in room air. Some rats were perfused with India ink before sacrifice and retinal dissection in order to study the effect of oxygen-rearing on the retinal vasculature. By this method it was found that oxygen-reared animals sustained a 36% loss of retinal blood vessels. Other animals' retinas were removed immediately after sacrifice and examined for evidence of lipid peroxidation by one of three means: 1) a determination of the presence of products of lipid peroxidation, 2) a measure of the loss of polyunsaturated fatty acids, and 3) a determination of retinal vitamin E level. Each of these determinations indicated that peroxidation reactions had occurred in the retinas of oxygenreared rats. Retinal vitamin E was supplemented in the young rats through the diet of the mothers. This treatment resulted in a two-fold increase of retinal vitamin E over levels in pups of mothers fed rat chow. Oxygen-reared vitamin E-supplemented rats sustained significantly less obliteration of blood vessels than non-supplemented oxygen-reared animals.
- Ashton N, Blach R. Studies in developing retinal vessels. VIII. Effect of oxygen on the vessels of the ratling. Br J Ophthalmol 1961; 45: 321–40.
- Ashton N, Pedler C. Studies on developing retinal vessels. IX. Reaction of endothelial cells to oxygen. Br J Ophthalmol 1962; 46: 257–76.
- Barber AA, Bernheim F. Lipid peroxidation: its measurement, occurrence, and significance in animal tissues. Adv Gerontol Res 1967; 2: 355–403.
- Bieri JC, Tolliver TJ, Gatignani GL. Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am J Clin Nutr 1979; 32: 2143–9.
- Bougle D, Vert P, Reichart E, Hartemann D, Heng EL. Retinal Superoxide dismutase activity in newborn kittens exposed to normobaric hyperoxia; effect of vitamin E. Pediatric Res 1982; 16: 400–2.
- Daemen FJM. Vertebrate rod outer segment membranes. Biochim Biophys Acta 1973; 300: 255–88.
- Delmelle M. Possible implication of photooxidation reactions in retinal photo-damage. Photochem Photobiol 1979; 29: 713–7.
- Flower RW, Blake DA. Retrolental fibroplasia: role of prostaglandin cascade in the pathogenesis of oxygen induced retinopathy in the newborn beagle. Pediat Res 1981; 15: 1293–1302.
- Hiramatsu T, Harata K, Nishigaki I, Yagi K. The formation of lipoperoxide in the retina of rabbits exposed to high concentration of oxygen. Experentia 1976; 32: 622–3. CrossRef
- Johnson L. Retrolental fibroplasia: a new look at an unsolved problem. Hosp Pract 1981; 16: 109–21.
- Patz A, Eastham A, Higgenbotham DH, Kleh T. Oxygen studies in retrolental fibroplasia. II. The production of the microscopic changes of retrolental fibroplasia in experimental animals. Am J Ophthalmol 1953; 36: 1511–22.
- Penn JS, Thum LA, Rhem MN, Dell SJ. Effects of oxygen rearing on the electroretinogram and GFA-protein in the rat. Invest Ophthalmol Vis Sci 1988; 29: 1623–30.
- Rodieck RW. The Vertebrate Retina. Principles of Structure and Function. San Francisco: W.H. Freeman and Co., 1973: 159.
- Taki M. Causal relationship of lipid peroxide with experimental retinopathy of prematurity. I. Morphological distribution of lipid peroxide in kitten retina after oxygen administration. Nagoya Med J 1983; 28: 115–20.
- Yagi K, Matsuoka S, Yagi K, Matsuoka S, Ohkawa H, Ohishi N, Takevchi Y, Kakai H. Lipoperoxide level of the retina of chick embryo exposed to high concentration of oxygen. Clin Chim Acta 1977; 355–60.
- Oxygen-induced retinopathy in the rat: Possible contribution of peroxidation reactions
Volume 74, Issue 3 , pp 179-186
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- Kluwer Academic Publishers
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- oxygen-induced retinopathy
- polyunsaturated fatty acid
- vitamin E
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- John S. Penn (1)
- Author Affiliations
- 1. Cullen Eye Institute, Baylor College of Medicine, 77030, Houston, TX, USA