Invertebrate Neuroscience

, Volume 3, Issue 2, pp 185–192

A subtractive cDNA library from an identified regenerating neuron is enriched in sequences up-regulated during nerve regeneration

  • Sergei Korneev
  • Alexei Fedorov
  • Rob Collins
  • Susanna E. Blackshaw
  • Jane A. Davies
Articles from the ICINN 97 Conference Neurogenesis and Regeneration

DOI: 10.1007/BF02480373

Cite this article as:
Korneev, S., Fedorov, A., Collins, R. et al. Invertebrate Neuroscience (1997) 3: 185. doi:10.1007/BF02480373
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Abstract

We have constructed a subtractive cDNA library from regenerating Retzius cells of the leech,Hirudo medicinalis. It is highly enriched in sequences up-regulated during nerve regeneration. Sequence analysis of selected recombinants has identified both novel sequences and sequences homologous to molecules characterised in other species. Homologies include α-tubulin, a calmodulin-like protein, CAAT/enhancer-binding protein (C/EBP), protein 4.1 and synapsin. These types of proteins are exactly those predicted to be associated with axonal growth and their identification confirms the quality of the library. Most interesting, however, is the isolation of 5 previously uncharacterised cDNAs which appear to be up-regulated during regeneration. Their analysis is likely to provide new information on the molecular mechanisms of neuronal regeneration.

Key Words

Retzius cell regeneration subtractive hybridisation cDNA library Hirudo medicinalis 

Copyright information

© Sheffield Academic Press 1997

Authors and Affiliations

  • Sergei Korneev
    • 1
  • Alexei Fedorov
    • 1
  • Rob Collins
    • 1
  • Susanna E. Blackshaw
    • 2
  • Jane A. Davies
    • 1
  1. 1.Sussex Centre for NeuroscienceSussex UniversityFalmerUK
  2. 2.Department of Human AnatomyUniversity of OxfordOxfordUK

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