Molecular and General Genetics MGG

, Volume 217, Issue 2, pp 430–436

Cloning, characterization and DNA sequencing of the gene encoding the Mr50000 quinoprotein glucose dehydrogenase fromAcinetobacter calcoaceticus

  • Anne-Marie Cleton-Jansen
  • Nora Goosen
  • Kees Vink
  • Pieter van de Putte
Article

DOI: 10.1007/BF02464914

Cite this article as:
Cleton-Jansen, A., Goosen, N., Vink, K. et al. Molec. Gen. Genet. (1989) 217: 430. doi:10.1007/BF02464914

Summary

Recently we described the cloning of the gene coding for a Mr 87000 glucose dehydrogenase (GDH-A) fromAcinetobacter calcoaceticus. In this report we describe the cloning of a gene coding for a second GDH (GDH-B) with a Mr of 50000 from the same organism. This gene was isolated using a 20-mer synthetic oligonucleotide, derived from the N-terminal amino acid sequence of purified GDH-B as a probe to screen a genomic bank. From the DNA sequence of thegdhB gene, a protein can be derived of Mr 52772 with a 24 amino acid signal peptide which is removed, resulting in the mature protein with a Mr 50231. In vitro transcription-translation of thegdhB clone shows the mature and the precursor protien. The derived amino acid sequence has no obvious homology with GDH-A ofA. calcoaceticus. We show that disaccharides are specific GDH-B substrates and that 2-deoxyglucose is specific for GDH-A.

Key words

Acinetobacter calcoaceticusDNA sequenceGlucose dehydrogenasePyrroloquinoline quinoneSignal sequence

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Anne-Marie Cleton-Jansen
    • 1
  • Nora Goosen
    • 1
  • Kees Vink
    • 1
  • Pieter van de Putte
    • 1
  1. 1.Laboratory of Molecular GeneticsUniversity of LeidenLeidenThe Netherlands