Article

Molecular and General Genetics MGG

, Volume 217, Issue 2, pp 411-418

Aminoglycoside suppression at UAG, UAA and UGA codons inEscherichia coli and human tissue culture cells

  • Robin MartinAffiliated withBiochemistry Laboratory, Biological Sciences, University of Sussex
  • , Anne E. MoggAffiliated withBiochemistry Laboratory, Biological Sciences, University of Sussex
  • , Louise A. HeywoodAffiliated withBiochemistry Laboratory, Biological Sciences, University of Sussex
  • , Lars NitschkeAffiliated withBiochemistry Laboratory, Biological Sciences, University of Sussex
  • , Julian F. BurkeAffiliated withBiochemistry Laboratory, Biological Sciences, University of Sussex

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Summary

We have compared the suppression of nonsense mutations by aminoglycoside antibiotics inEscherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl transferase (cat) gene, in the vector pRSVcat, were suppressed by growth in G418 and paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme assays for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and during transient expression from the same plasmid in human 293 tissue culture cells. We have found significant differences in the degree of suppression amongst three UAG codons and two UAA codons in different mRNA contexts. However, the pattern of these effects are not the same in the two organisms. Our data suggest that context effects of nonsense suppression may operate under different rules inE. coli and human cells.

Key words

Nonsense suppression Context effects Aminoglycosides