The paromomycin resistance mutation (parr-454) in the 15 S rRNA gene of the yeastSaccharomyces cerevisiae is involved in ribosomal frameshifting
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- Weiss-Brummer, B. & Hüttenhofer, A. Molec. Gen. Genet. (1989) 217: 362. doi:10.1007/BF02464905
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The leaky expression of the yeast mitochondrial geneoxi1, containing a frameshift mutation (+1), is caused by natural frameshift suppression, as shown previously (Fox and Weiss-Brummer 1980). A drastic decrease in the natural level of frameshifting is found in the presence of theparr-454 mutation, localized at the 3′ end of the 15 S rRNA gene. This mutation causes resistance to the antibiotic paronomycin in the yeast strains D273-10B and KL14-4A (Li et al. 1982; Tabak et al. 1982). The results of this study imply that in the yeast strain 777-3A this mutation alone is sufficient for restriction of the level of natural frameshifting but is insufficient to confer resistance to paromomycin. A second mutation, arising spontaneously with a frequency of 10−4 leads, in combination with theparr-454 mutation, to full paromomycin resistance in strain 777-3A.