Original Papers


, Volume 42, Issue 5, pp 447-453

First online:

Ribosomal DNA-ITS sequence polymorphism in the sugarcane rust,Puccinia kuehnii

  • Eric V. VirtudazoAffiliated withInstitute of Agriculture and Forestry, University of Tsukuba
  • , Hitoshi NakamuraAffiliated withNational Institute of Agro-Environmental Sciences
  • , Makoto KakishimaAffiliated withInstitute of Agriculture and Forestry, University of Tsukuba Email author 

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The two highly divergent ITS types previously observed in isolates ofPuccinia kuehnii were amplified from the same isolates through the use of ITS type-specific primers for PCR and are therefore considered as polymorphisms of ITS regions within the species. Although homology of sequences of one of the ITS types with otherPuccinia species was of expected levels, significantly high homology of sequences of the other type with those ofCronartium members indicates that abnormal genetic events led to the occurrence of these polymorphic regions. These results indicate that ITS gene tree phylogeny may not reflect true species phylogeny in this group of rust fungi. Rather, D1/D2 region tree phylogeny, which was concordant with the differences in morphology with and among related rusts, more correctly reflects phylogenetic relationships of sugarcane and related grass rusts.

Key Words

Puccinia kuehnii ribosomal DNA ITS polymorphism sugarcane rust