Biotechnology Techniques

, Volume 6, Issue 6, pp 527–532

Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods

Authors

  • Yasuyuki Sakai
    • Insitute of Industrial ScienceUniversity of Tokyo
  • Katsuko Furukawa
    • Department of Industrial ChemistryMeiji University
  • Motoyuki Suzuki
    • Insitute of Industrial ScienceUniversity of Tokyo
Article

DOI: 10.1007/BF02447826

Cite this article as:
Sakai, Y., Furukawa, K. & Suzuki, M. Biotechnol Tech (1992) 6: 527. doi:10.1007/BF02447826

Summary

Spheroidal aggregates (spheroids) of adult rat hepatocytes in primary culture formed by rotational tissue culture methods. The time suitable for terminating the rotation and reseeding was determined. The spheroids formed were immobilized stably onto polylysine-coated surfaces, and they secreted albumin for about 2 weeks at almost the same level as compared to spheroids formed by stationary culture.

Copyright information

© Science & Technology Letters 1992