Biotechnology Techniques

, Volume 8, Issue 12, pp 901–904

Transformation ofLeuconostoc oenos by electroporation

  • Leon M. T. Dicks

DOI: 10.1007/BF02447736

Cite this article as:
Dicks, L.M.T. Biotechnol Tech (1994) 8: 901. doi:10.1007/BF02447736


An electroporation-induced transformation system was developed forLeuconostoc oenos. Of the three plasmids tested (pGK13, pAMS100 and pIL253), only pGK13 was expressed. Several parameters were studied with possible influence on transformation efficiency. Electroporation conditions of 2000 V/cm, 25μF and 200 ohm proved successful. Transformation efficiencies (number of cells/μg plasmid DNA) of 1 X 103 were obtained.

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Leon M. T. Dicks
    • 1
  1. 1.Department of MicrobiologyUniversity of StellenboschStellenboschSouth Africa