Mammalian Genome

, Volume 1, Issue 2, pp 78–83

Methylation status of CpG-rich islands on active and inactive mouse X chromosomes

  • Dominic P. Norris
  • Neil Brockdorff
  • Sohaila Rastan
Original Contributions

DOI: 10.1007/BF02443782

Cite this article as:
Norris, D.P., Brockdorff, N. & Rastan, S. Mammalian Genome (1991) 1: 78. doi:10.1007/BF02443782

Abstract

Single copy probes derived from CpG-rich island clones fromEag I andNot I linking libraries and nine rare-cutter restriction endonucleases were used to investigate the methylation status of CpG-rich islands on the inactive and active X chromosomes (Chr) of the mouse. Thirteen of the 14 probes used detected CpG-rich islands in genomic DNA. The majority of island CpGs detected by rare-cutter restriction endonucleases were methylated on the inactive X Chr and unmethylated on the active X Chr, but some heterogeneity within the cell population used to make genomic DNA was detected. The CpG-rich islands detected by two putative pseudoautosomal probes remained unmethylated on both the active and inactive X Chrs. Otherwise, distance from the X Chr inactivation center did not affect the methylation profile of CpG-rich islands. We conclude that methylation of CpG-rich islands is a general feature of X Chr inactivation.

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Dominic P. Norris
    • 1
  • Neil Brockdorff
    • 1
  • Sohaila Rastan
    • 1
  1. 1.Section of Comparative BiologyMRC Clinical Research CentreHarrowUK

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