Letters in Peptide Science

, Volume 10, Issue 5, pp 621–629

Chitosan-dextran sulfate nanoparticles for delivery of an anti-angiogenesis peptide

Article

DOI: 10.1007/BF02442596

Cite this article as:
Chen, Y., Mohanraj, V.J. & Parkin, J.E. Lett Pept Sci (2003) 10: 621. doi:10.1007/BF02442596

Summary

A novel nanoparticle delivery system has been developed by employing the oppositely charged polymers chitosan (CS) and dextran sulfate (DS), and a simple coacervation process. Under the conditions investigated, the weight ratio of the two polymers is identified as a determining factor controlling particle size, surface charge, entrapment efficiency and release characteristics of the nanoparticles produced. Particles of 223 nm mean diameter were produced under optimal conditions with a zeta potential of approximately −32.6 mV. A maximum of 75% anti-angiogenesis peptide entrapment efficiency was achieved with a CS:DS weight ratio of 0.59∶1. The same nanoparticle formulation also showed slow and sustained peptide release over a period of 6 days. In contrast, the formulation containing a lower ratio of CS:DS (0.5∶1) was found to have reduced entrapment efficiency and more rapid peptide release characteristics. The results of this study suggest that physicochemical and release characteristics of the CS-DS nanoparticles can be modulated by changing ratios of two ionic polymers. The novel CS-DS nanoparticles prepared by the coacervation process have potential as a carrier for small peptides.

Key words

chitosandextran sulphatenanoparticlespeptide deliverysustained-release

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Yan Chen
    • 1
    • 2
  • Vellore J. Mohanraj
    • 3
  • John E. Parkin
    • 2
  1. 1.Western Australian Biomedical Research Institute, School of PharmacyCurtin UniversityPerth
  2. 2.School of PharmacyCurtin UniversityPerth
  3. 3.Fourrts (India) Laboratories Private LimitedChennaiIndia