Histochemistry

, Volume 94, Issue 2, pp 211–215

Immunohistochemical demonstration of fibre type-specific isozymes of cytochrome c oxidase in human skeletal muscle

  • N. Romero
  • C. Marsac
  • M. Fardeau
  • M. Droste
  • B. Schneyder
  • B. Kadenbach
Article

DOI: 10.1007/BF02440190

Cite this article as:
Romero, N., Marsac, C., Fardeau, M. et al. Histochemistry (1990) 94: 211. doi:10.1007/BF02440190

Summary

The immunohistochemical reaction of monoclonal as well as polyclonal antibodies against cytochrome c oxidase (COX) subunits with serial sections of normal human skeletal muscle was investigated. The stronger reactivity of polyclonal antibodies to COX subunits II–III and VIIbc with type I as compared to type II fibres, correlated well with the higher histochemical reactivity of NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase in type I fibres. In contrast an almost exclusive reaction of a monoclonal antibody against subunit IV with type I fibre and a preponderan reaction of a polyclonal antibody against subunits Vab with type II fibres was obtained. Antibodies against subuntis I, Vb and VIc did not reveal a fibre-type-specific reactivity. The data indicate in human muscle the occurrence of fibre type-specific isozymes of cytochrome c oxidase differing in subunits IV and Va or Vb.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • N. Romero
    • 1
  • C. Marsac
    • 2
  • M. Fardeau
    • 1
  • M. Droste
    • 3
  • B. Schneyder
    • 3
  • B. Kadenbach
    • 3
  1. 1.Unite de Recherche Biologie et Pathologie Neuromusculaires INSERM U 153CNRS UA 614ParisFrance
  2. 2.Faculte de Medecine Necker Enfants MaladesLaboratoire de Biochimie, INSERM U. 75Paris, Cedex 15France
  3. 3.Fachbereich Chemie, BiochemiePhilipps-UniversitätMarburgFederal Republic of Germany