Biotechnology Techniques

, Volume 5, Issue 4, pp 299–302

Formation of spheroids of adult rat hepatocytes on polylysine-coated surfaces and thier albumin production

Authors

  • Yasuyuki Sakai
    • Institute of Industrial ScienceUniversity of Tokyo
  • Motoyuki Suzuki
    • Institute of Industrial ScienceUniversity of Tokyo
Article

DOI: 10.1007/BF02438667

Cite this article as:
Sakai, Y. & Suzuki, M. Biotechnol Tech (1991) 5: 299. doi:10.1007/BF02438667

Summary

Adult rat hepatocytes in primary culture formed spheroidal aggregates on the surfaces precoated with more than 0.01 mg-polylysine/mL. Epidermal growth factor (EGF) improved the formation and the maintenance of spheroid. The albumin secretion of the spheroids formed was maintained about 2 weeks at a high level.

Copyright information

© Science & Technology Letters 1991