The Journal of Membrane Biology

, Volume 4, Issue 1, pp 227–251

The effect of uncouplers of oxidative phosphorylation on lipid bilayer membranes: Carbonylcyanidem-chlorophenylhydrazone

  • Oliver H. LeBlancJr.
Article

DOI: 10.1007/BF02431973

Cite this article as:
LeBlanc, O.H. J. Membrain Biol. (1971) 4: 227. doi:10.1007/BF02431973

Summary

Detailed experimental data for conductivity and membrane potentials are presented for lecithin/cholesterol/decane bilayers in the presence of the uncoupler carbonylcyanidem-chlorophenylhydrazone (CCCP). These compare favorably with a theoretical model derived to explain the mechanism of action of uncouplers on bilayers. The model assumes that the weak acid uncoupler HA and its anion A are the sole species which permeate the membrane. Its key feature is the recognition of the existence of unstirred aqueous layers on either side of the membrane. The model accounts for, among other things, a maximum in the transmembrane conductivity at a pH to the alkaline side of the uncoupler pKa and saturating current-voltage characteristics at high pH, both phenomena being found for CCCP. From a quantitative fit of model to data, values of 2.0×10−3 and 11 cm/sec are deduced for the permeability coefficients of the CCCP anion and the undissociated CCCP molecule, respectively.

Copyright information

© Springer-Verlag New York Inc 1971

Authors and Affiliations

  • Oliver H. LeBlancJr.
    • 1
  1. 1.General Electric Research and Development CenterSchenectady