Applied Microbiology and Biotechnology

, Volume 43, Issue 5, pp 877–879

CEN14 sequences cause slower proliferation, reduced cell size and asporogeny inSaccharomyces cerevisiae

  • J. R. Dickinson
  • J. M. Wingfield
  • D. J. Mason
Short Contribution

DOI: 10.1007/BF02431922

Cite this article as:
Dickinson, J.R., Wingfield, J.M. & Mason, D.J. Appl Microbiol Biotechnol (1995) 43: 877. doi:10.1007/BF02431922

Abstract

The introduction ofCEN14-based plasmids into haploid strains ofSaccharomyces cerevisiae resulted in reduced proliferation rates and significantly smaller (20%) cell size than in untransformed control cells. This could be useful to those yeast biotechnology processes that require high levels of gene expression but little or no yeast growth and proliferation. In diploids similar plasmids caused asporogeny, which was possibly a consequence of the reduced cell size.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • J. R. Dickinson
    • 3
  • J. M. Wingfield
    • 3
  • D. J. Mason
    • 3
  1. 1.Yamanouchi Research InstituteLittlemore HospitalOxfordUK
  2. 2.Division of Microbiology, UMDSSt Thomas' HospitalLondonUK
  3. 3.School of Pure and Applied BiologyUniversity of Wales, College of CardiffCardiffUK