Molecular cloning and chromosomal assignment of a human perforin (PFP) gene
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- Shinkai, Y., Yoshida, M.C., Maeda, K. et al. Immunogenetics (1989) 30: 452. doi:10.1007/BF02421177
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Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome17q11-21.