Immunogenetics

, Volume 30, Issue 6, pp 452–457

Molecular cloning and chromosomal assignment of a human perforin (PFP) gene

Authors

  • Yoichi Shinkai
    • Department of ImmunologyJuntendo University School of Medicine
  • Michihiro C. Yoshida
    • Chromosome Research Unit, Faculty of ScienceHokkaido University
  • Keiko Maeda
    • Department of ImmunologyJuntendo University School of Medicine
  • Tetsuji Kobata
    • Department of ImmunologyJuntendo University School of Medicine
  • Kazuo Maruyama
    • Department of Molecular BiologyDNAX Research Institute of Molecular and Cellular Biology
  • Junji Yodoi
    • Institute for Immunology and Department of Pediatrics, Faculty of MedicineKyoto University
  • Hideo Yagita
    • Department of ImmunologyJuntendo University School of Medicine
  • Ko Okumura
    • Department of ImmunologyJuntendo University School of Medicine
Article

DOI: 10.1007/BF02421177

Cite this article as:
Shinkai, Y., Yoshida, M.C., Maeda, K. et al. Immunogenetics (1989) 30: 452. doi:10.1007/BF02421177

Abstract

Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome17q11-21.

Copyright information

© Springer-Verlag 1989