Article

Immunogenetics

, Volume 30, Issue 6, pp 452-457

First online:

Molecular cloning and chromosomal assignment of a human perforin (PFP) gene

  • Yoichi ShinkaiAffiliated withDepartment of Immunology, Juntendo University School of Medicine
  • , Michihiro C. YoshidaAffiliated withChromosome Research Unit, Faculty of Science, Hokkaido University
  • , Keiko MaedaAffiliated withDepartment of Immunology, Juntendo University School of Medicine
  • , Tetsuji KobataAffiliated withDepartment of Immunology, Juntendo University School of Medicine
  • , Kazuo MaruyamaAffiliated withDepartment of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology
  • , Junji YodoiAffiliated withInstitute for Immunology and Department of Pediatrics, Faculty of Medicine, Kyoto University
  • , Hideo YagitaAffiliated withDepartment of Immunology, Juntendo University School of Medicine
  • , Ko OkumuraAffiliated withDepartment of Immunology, Juntendo University School of Medicine

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Abstract

Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome17q11-21.