Calcified Tissue International

, Volume 34, Issue 1, pp 291–294

Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers

  • D. J. Simmons
  • G. N. Kent
  • R. L. Jilka
  • D. M. Scott
  • M. Fallon
  • David V. Cohn
Laboratory Investigations

DOI: 10.1007/BF02411253

Cite this article as:
Simmons, D.J., Kent, G.N., Jilka, R.L. et al. Calcif Tissue Int (1982) 34: 291. doi:10.1007/BF02411253

Summary

Osteoblast-like and osteoclast-like cells freed from neonatal calvaria by sequential enzymatic digestion after 6–7 days in culture were placed in diffusion chambers and implanted in the peritoneal cavities of CD-1 mice. About half of the chambers also contained a dead calvarium to test for the need of an “inducer.” After 20 days, 11 of 18 chambers containing the osteoblast-like cells formed large foci of mineralized bone that corresponded to alkaline phosphatase activity throughout the chambers. Moreover, only type I (i.e., bone) collagen was formed. Occasional deposits of bone were found in only 3 of 22 chambers containing the osteoclast-like cells. The presence of dead bone did not affect any of the results. These data confirm the osteoblast-like nature of the isolated cell populations and demonstrate that these cells retain their differentiated function in culture.

Key words

Bone formation Osteoblasts Osteoclasts Bone Induction Alkaline Phosphatase Cell Culture 

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • D. J. Simmons
    • 1
  • G. N. Kent
    • 2
  • R. L. Jilka
    • 2
    • 3
  • D. M. Scott
    • 2
  • M. Fallon
    • 4
  • David V. Cohn
    • 2
    • 3
  1. 1.Division of Orthopedic SurgeryWashington UniversitySt. Louis
  2. 2.Research LaboratoriesV.A. Medical CenterKansas City
  3. 3.University of Kansas Medical CenterKansas City
  4. 4.Department of PathologyJewish Hospital of St. LouisSt. LouisUSA

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