Calcified Tissue International

, Volume 34, Issue 1, pp 291–294

Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers


  • D. J. Simmons
    • Division of Orthopedic SurgeryWashington University
  • G. N. Kent
    • Research LaboratoriesV.A. Medical Center
  • R. L. Jilka
    • Research LaboratoriesV.A. Medical Center
    • University of Kansas Medical Center
  • D. M. Scott
    • Research LaboratoriesV.A. Medical Center
  • M. Fallon
    • Department of PathologyJewish Hospital of St. Louis
  • David V. Cohn
    • Research LaboratoriesV.A. Medical Center
    • University of Kansas Medical Center
Laboratory Investigations

DOI: 10.1007/BF02411253

Cite this article as:
Simmons, D.J., Kent, G.N., Jilka, R.L. et al. Calcif Tissue Int (1982) 34: 291. doi:10.1007/BF02411253


Osteoblast-like and osteoclast-like cells freed from neonatal calvaria by sequential enzymatic digestion after 6–7 days in culture were placed in diffusion chambers and implanted in the peritoneal cavities of CD-1 mice. About half of the chambers also contained a dead calvarium to test for the need of an “inducer.” After 20 days, 11 of 18 chambers containing the osteoblast-like cells formed large foci of mineralized bone that corresponded to alkaline phosphatase activity throughout the chambers. Moreover, only type I (i.e., bone) collagen was formed. Occasional deposits of bone were found in only 3 of 22 chambers containing the osteoclast-like cells. The presence of dead bone did not affect any of the results. These data confirm the osteoblast-like nature of the isolated cell populations and demonstrate that these cells retain their differentiated function in culture.

Key words

Bone formationOsteoblastsOsteoclastsBone InductionAlkaline PhosphataseCell Culture

Copyright information

© Springer-Verlag 1982