Calcified Tissue International

, Volume 33, Issue 1, pp 255–260

Uptake of collagenolytic enzymes by bone cells during isolation from embryonic rat calvaria

Authors

  • Jaro Sodek
    • Medical Research Council Group in Periodontal Physiology, Faculty of DentistryUniversity of Toronto
  • Johan N. M. Heersche
    • Medical Research Council Group in Periodontal Physiology, Faculty of DentistryUniversity of Toronto
Article

DOI: 10.1007/BF02409446

Cite this article as:
Sodek, J. & Heersche, J.N.M. Calcif Tissue Int (1981) 33: 255. doi:10.1007/BF02409446

Summary

Collagenolytic activity extracted from bone cells, isolated from calvaria with a proteolytic enzyme mixture, was analyzed using14C-labeled collagen and a combination of gel electrophoresis and fluorography. The pattern of collagen degradation products obtained did not include 3/4-fragments, typical of vertebrate collagenases, but was essentially identical to the patterns generated by very dilute samples of the proteolytic enzyme mixture used to isolate the bone cells, and purified collagenase fromClostridium histolyticum (E.C. 3.4.24.3.). These and other results strongly suggest that the previously reported collagenolytic activity of bone cells [1] is exogenous in origin.

Key words

Bacterial collagenaseVertebrate collagenaseSodium dodecyl sulfate polyacrylamide gel electrophoresisFluorography

Copyright information

© Springer-Verlag 1981