Clinical Investigations

Calcified Tissue International

, Volume 36, Issue 1, pp 77-82

First online:

Assessment of anin vivo diffusion chamber method as a quantitative assay for osteogenesis

  • I. BabAffiliated withMRC Bone Research Laboratory, Nuffield Orthopaedic Centre
  • , B. A. AshtonAffiliated withMRC Bone Research Laboratory, Nuffield Orthopaedic Centre
  • , G. T. SyftestadAffiliated withMRC Bone Research Laboratory, Nuffield Orthopaedic Centre
  • , M. E. OwenAffiliated withMRC Bone Research Laboratory, Nuffield Orthopaedic Centre

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Summary

The alkaline phosphatase activity and the calcium and phosphorus content of osteogenic tissue formedin vivo following the implantation of diffusion chambers loaded with rabbit bone marrow cells is reported. (In this study the term osteogenic includes osteoblastic and chondroblastic.) Chambers examined 14–70 days after implantation revealed progressive accumulation of mineral. Alkaline phosphatase activity increased until day 30 and declined thereafter. The osteogenic potential of the marrow cells decreased with increasing weight (age) of the cell donor rabbit when measured either as the percentage of chambers containing osteogenic tissue or as the amount of calcium, phosphorus, or alkaline phosphatase activity within the chambers. The results confirm that measurements of these parameters in tissue formed by cells incubated in diffusion chambersin vivo may be used as a method for assay of osteogenesis.

Key words

Osteogenesis Diffusion chambers Alkaline phosphatase Calcium Phosphorus