Calcified Tissue International

, Volume 35, Issue 1, pp 177–182

Autoradiographic localization of target cells for 1α, 25-dihydroxyvitamin D3 in bones from fetal rats

  • R. Narbaitz
  • W. E. Stumpf
  • M. Sar
  • S. Huang
  • H. F. DeLuca
Laboratory Investigations

DOI: 10.1007/BF02405028

Cite this article as:
Narbaitz, R., Stumpf, W.E., Sar, M. et al. Calcif Tissue Int (1983) 35: 177. doi:10.1007/BF02405028

Summary

Thaw-mount autoradiographic studies after injection of3H-1,25-D3 were conducted on 18-and 20-day-old rat fetuses. In maxillary bones, ribs, and tibia, nuclear concentration of radioactivity was found in osteoprogenitor cells and osteoblasts. Osteocytes and chondrocytes in epiphyseal plates were either unlabeled or weakly labeled. In competition experiments, nuclear concentration of radioactivity was blocked by the injection of a high dose of nonradioactive 1,25-D3 prior to the administration of the labeled hormone, but not by a similar dose of nonradioactive 25-D3. The results are interpreted as indicating that osteoprogenitor cells and osteoblasts are target cells for the direct action of 1,25-D3 on fetal bone.

Key words

Vitamin DBoneOsteoblastsReceptors1,25-dihydroxyvitamin D3

Copyright information

© Springer-Verlag New York Inc. 1983

Authors and Affiliations

  • R. Narbaitz
    • 1
  • W. E. Stumpf
    • 2
  • M. Sar
    • 2
  • S. Huang
    • 2
  • H. F. DeLuca
    • 3
  1. 1.Department of AnatomyUniversity of OttawaOttawaCanada
  2. 2.Department of AnatomyUniversity of North CarolinaChapel HillUSA
  3. 3.Department of BiochemistryUniversity of WisconsinMadisonUSA