Journal of tissue culture methods

, Volume 15, Issue 4, pp 171–175

Isolation and characterization of chicken aortic endothelial cells

  • Hana Van Campen
  • Maria R. Davis
Article

DOI: 10.1007/BF02388315

Cite this article as:
Van Campen, H. & Davis, M.R. Journal of Tissue Culture Methods (1993) 15: 171. doi:10.1007/BF02388315

Summary

The isolation of endothelial cells from the aortae of chickens and maintenance in culture is described. The uptake of acetylated low-density lipoprotein labeled with 1,1′-dioctadecyl-1,3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate was used to purify the endothelial cells by fluorescence-activated cell sorting (FACS). The morphology of the purified cells is similar to that of endothelial cells from other species; however, these characteristics change rapidly with continued culture and passage.

Key words

chickenavianendothelialaortaecell cultureFACS

Copyright information

© Tissue Culture Association 1993

Authors and Affiliations

  • Hana Van Campen
    • 1
  • Maria R. Davis
    • 1
  1. 1.Department of Veterinary ScienceThe Pennsylvania State UniversityUniversity Park