Contributed Papers

Journal of tissue culture methods

, Volume 13, Issue 4, pp 275-283

First online:

Cryopreservation of embryonic chick myogenic lineage-committed stem cells

  • Henry E. YoungAffiliated withDivision of Basic Medical Sciences, Mercer University School of MedicineDepartment of Surgery, Mercer University School of Medicine
  • , Donna C. MorrisonAffiliated withDivision of Basic Medical Sciences, Mercer University School of Medicine
  • , Jonathan D. MartinAffiliated withDivision of Basic Medical Sciences, Mercer University School of Medicine
  • , Paul A. LucasAffiliated withDepartment of Surgery, Mercer University School of MedicineDepartment of Surgery, Medical Center of Central Georgia

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Summary

Undifferentiated embryonic chick stem cells provide a good in vitro test system to examine the effects of recombinant growth factors on the resultant phenotypic expression of these cells. One of the major difficulties in using freshly isolated cells is variation in the proportion of stem cells recovered from different cell harvests. Variation is of particular concern when multiple cell harvests are necessary to provide cells for assaying a single growth factor. As an attempt to minimize this variation, we have examined the efficacy of cryopreserving myogenic lineage-committed stem cells derived from embryonic chick leg muscle. Percent recovery, viability, differentiation morphology, and cellular proliferation rates were compared between sister cultures of freshly isolated and cryopreserved stem cells. Embryonic chick stem cells retained their capacity to differentiate myogenically in culture when slowly frozen in and recovered from 7.5% dimethyl sulfoxide medium supplemented with horse serum and stage-specific embryo extract.

Key words

cryopreservation embryonic chick myogenic stem cells