Annals of Biomedical Engineering

, Volume 21, Issue 1, pp 77–83

Design and construction of a linear shear stress flow chamber

Authors

  • Shunichi Usami
    • Institute of Biomedical SciencesAcademia Sinica
  • Hsuan-Hsu Chen
    • Institute of Biomedical SciencesAcademia Sinica
  • Yihua Zhao
    • Department of Applied Mechanics and Engineering Sciences/BioengineeringUniversity of California
  • Shu Chien
    • Department of Applied Mechanics and Engineering Sciences/BioengineeringUniversity of California
  • Richard Skalak
    • Department of Applied Mechanics and Engineering Sciences/BioengineeringUniversity of California
Article

DOI: 10.1007/BF02368167

Cite this article as:
Usami, S., Chen, H., Zhao, Y. et al. Ann Biomed Eng (1993) 21: 77. doi:10.1007/BF02368167

Abstract

A new paralle plate flow chamber that has a linear variation of shear stress, starting from a predetermined maximum value at the entrance and falling to zero at the exit, has been designed and tested. This is in contrast to the usual rect-angular channel plan which produces a constant shear stress over the entire length. The new design is based on the theory of Hele-Shaw flow between parallel plates. To verify the efficacy of the flow channel, the effect of fluid shear stress on platelet adhesion to a fibrinogen-coated glass surface was tested. The percentage of attached platelets after 5 min of shear stress is shown to be a function of shear stress. With this new flow chamber, cell-cell interactions can be studied efficiently over a wide range of shear stress using a single run at constant discharge.

Keywords

Flow chamberHele-Shaw flowCell adhesion

Copyright information

© Pergamon Press Ltd. 1993