Behavior Genetics

, Volume 26, Issue 1, pp 55–63

Comparative diagnoses of twin zygosity by SSLP variant analysis, questionnaire, and dermatoglyphic analysis

  • Elisabeth Spitz
  • René Moutier
  • Terry Reed
  • Marie Claire Busnel
  • Catherine Marchaland
  • Pierre L. Roubertoux
  • Michèle Carlier
Article

DOI: 10.1007/BF02361159

Cite this article as:
Spitz, E., Moutier, R., Reed, T. et al. Behav Genet (1996) 26: 55. doi:10.1007/BF02361159

Abstract

Zygosity diagnosis has been performed in 79 pairs of twins using three methods. Simple sequence repeat length polymorphism (SSLP) analysis allows an efficient classification (MZ or DZ) with only a few markers following a simplified technique of extraction and amplification. A method based on a full questionnaire completed by parents about twin similarity correctly classifies 97.46% of the pairs; 92.41% are correctly classified using only four questions as suggested by logistic regression analysis. The third method, using dermatoglyphic analyses, correctly classifies 86.76% of pairs. To lower the cost of DNA diagnosis we stress the possibility of limiting its use to pairs with scores in the overlap area between MZ and DZ twins with a validated questionnaire.

Key Words

Dermatoglyphicsmicrosatellitestwins zygosityzygosity questionnaire

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Elisabeth Spitz
    • 1
  • René Moutier
    • 1
  • Terry Reed
    • 2
  • Marie Claire Busnel
    • 1
  • Catherine Marchaland
    • 1
  • Pierre L. Roubertoux
    • 1
  • Michèle Carlier
    • 1
  1. 1.URA 1294 CNRS Génétique Neurogénétique et ComportementUniversité Paris V René Descartes, UFR BiomédicaleParis Cedex 06France
  2. 2.Department of Medical and Molecular GeneticsIndiana UniversityIndianapolis