Characterization of pectic substances from two potato cultivars with different sensitivities to prewarming
Potato cultivars Chieftain and Kennebec were prewarmed at 70°C for 15 min. prior to heating at 100°C for 18 min. in a water bath. Chieftain exhibited a large increase in fracturability upon treatment while Kennebec showed a smaller increase. Pectic substances of the two cultivars were sequentially extracted in water, sodium hexametaphosphate (0.5%), hydrochloric acid (0.05M, 50°C), and sodium hydroxide (0.05M, 5°C). The largest fraction isolated from both cultivars consisted of hydroxide-soluble pectic substances, and was followed by the acid-soluble fraction. Chieftain contained significantly greater amounts of the hydroxide-soluble fraction than Kennebec. Ion exchange chromatography revealed that the extracted pectic substances from the two major fractions consisted of a charged pectin chain with attached neutral sugars. Gel filtration chromatography revealed wide molecular size distributions for the isolated pectic substances. Pectic neutral sugar content for the hydroxide-soluble fraction (50% of total pectic substances) was greater for Chieftain than for Kennebec.
- Ahmed, E.R.A. & J.M. Labavitch, 1977. A simplified method for accurate determination of cell wall uronide, content.Journal of Food Biochemistry 1:361–365.
- Barbier, M. & J.F. Thibault, 1982. Pectic substances of cherry fruits.Phytochemistry 21: 111–115.
- Bartolome, L.G. & J.E. Hoff, 1972. Firming of potatoes. Biochemical effects of preheating.Journal of Agricultural and Food Chemistry 20: 266–270.
- Bettelheim, F.A. & C. Sterling, 1955. Factors associated with potato texture. II. Pectic substances.Food Research 20: 118–129.
- Blumenkrantz, N. & G. Asboe-Hansen, 1973. A new method for quantitative determination of uronic acids,Analytical Biochemistry 54: 484–489. CrossRef
- Buren, J.P. van. 1979. The chemistry of texture in fruits and vegetables.Journal of Texture Studies 10: 1–23.
- DuBois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers & F. Smith, 1956. Colorimetric method for determination of sugars and related substances.Analytical Chemistry 28(3): 350–356. CrossRef
- Hudson, J.M. & R.W. Buescher, 1985. Pectic substances and firmness of cucumber pickles as influenced by CaCl2, NaCl, and brine storage,Journal of Food Biochemistry 9: 211–229.
- Ishii, S., 1981. Isolation and characterization of cell-wall pectic substances from potato tuber.Phytochemistry 20(10): 2329–2333. CrossRef
- Jarvis, M.C., 1984. Structure and properties of pectin gels in plant cell walls.Plant, Cell and Environment 7: 153–164.
- Keegstra, K., K.W. Talmadge, W.D. Bauer & P. Albersheim, 1973. The structure of plant cell walls. III. A model of the walls of suspension-cultured sycamore cells based on the interconnections of the macromolecular components.Plant Physiology 51: 188–196.
- Keijbets, M.J.H. & W. Pilnik 1974. Some problems in the analysis of pectin in potato tuber tissue,Potato Research 17: 169–177.
- Loh, J. & W.M. Breene, 1982. Between-species differences in fracturability loss: comparison of the thermal behavior of pectic and cell wall substances in potato and Chinese waterchestnut.Journal of Texture Studies 13: 381–396.
- Moledina, K.H., M. Haydar, B. Ooraikul & D. Hadziyev, 1981. Pectin changes in the precooking step of dehydrated mashed potato production.Journal of the Science of Food and Agriculture 32: 1091–1102.
- Oehlert, G.W., 1989. MacAnova, version 2.0. Technical Report #493, University of Minnesota, School of Statistics.
- Plat, D., N. Ben-Shalom, A. Levi, D. Reid & E.E. Goldschmidt, 1988. Degradation of pectic substances in carrots by heat treatment,Journal of Agricultural and Food Chemistry 36: 362–365. CrossRef
- Saulnier, L. & J.F. Thibault, 1987. Extraction and characterization of pectic substances from pulp of grape berries.Carbohydrate Polymers 7: 329–343.
- Taguchi, M., H.W. Schafer & W.M. Breene, 1991. Influence of cultivar and prewarming on texture retention of thermally processed potatoesPotato Research 34: 29–39.
- Talmadge, K.W., K. Keegstra, W.D. Bauer & P. Albersheim, 1973. The structure of plant cell walls. I. The macromolecular components of the walls of suspension-cultured, sycamore cells with a detailed analysis of the pectic polymers.Plant Physiology 51: 158–173.
- Vries, J.A. de A.G.J. Voragen, F.M. Rombouts & W. Pilnik, 1984. Changes in the structure of apple pectic substances during ripening and storage.Carbohydrate Polymers 4:3–13.
- Wahem, I.A., 1988. The influence of the firmness of raw tomatoes on the physical, chemical, and sensory characteristics of canned products.Journal of Food Quality 11: 107–116.
- Characterization of pectic substances from two potato cultivars with different sensitivities to prewarming
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