Journal of Molecular Evolution

, Volume 42, Issue 5, pp 537–542

Phylogenetic analysis of the isopenicillin-N-synthetase horizontal gene transfer

Authors

  • Celia Buades
    • Departamento de Genética, Facultad de Biología and Servicio de BioinfonnáticaUniversidad de Valencia
  • Andrés Moya
    • Departamento de Genética, Facultad de Biología and Servicio de BioinfonnáticaUniversidad de Valencia
Articles

DOI: 10.1007/BF02352283

Cite this article as:
Buades, C. & Moya, A. J Mol Evol (1996) 42: 537. doi:10.1007/BF02352283

Abstract

A phylogenetic study of the isopenicillin-N-synthetase (IPNS) gene sequence from prokaryotic and lower eukaryotic producers of β-lactam antibiotics by means of a maximum-likelihood approach has been carried out. After performing an extensive search, rather than invoking a global molecular clock, the results obtained are best explained by a model with three rates of evolution. Grouped in decreasing order, these correspond toA. nidulans and then to the rest of the eukaryotes and prokaryotes, respectively. The estimated branching date between prokaryotic and fungal IPNS sequences (852 ±106 MY) strongly supports the hypothesis that the IPNS gene was horizontally transferred from bacterial β-lactam producers to filamentous fungi.

Key words

Horizontal gene transferβ-lactam producersMaximum likelihoodMolecular clock

Copyright information

© Springer-Verlag New York Inc 1996