Ichthyological Research

, Volume 43, Issue 2, pp 117–124

Genetic markers distinguishing between the two subspecies of the rosy bitterling,Rhodeus ocellatus (Cyprinidae)

  • Yoshikazu Nagata
  • Tadashi Tetsukawa
  • Takanori Kobayashi
  • Ken-ichi Numachi

DOI: 10.1007/BF02348234

Cite this article as:
Nagata, Y., Tetsukawa, T., Kobayashi, T. et al. Ichthyological Research (1996) 43: 117. doi:10.1007/BF02348234


Eleven populations of the rosy bitterling,Rhodeus ocellatus, from different localities in Japan, were genetically compared at 16 protein-coding loci using starch-gel electrophoresis. Two loci,Ldh-2 andPgdh, were demonstrated as diagnostic markers for the identification of two subspecies;R. ocellatus kurumeus, which is native to Japan, andR. ocellatus ocellatus, which was introduced from China. The two subspecies were distinguished by the complete substitution of different alleles between them. Population ofR. ocellatus kurumeus occurring in Yao City, Osaka, and in Kanzaki, Saga Prefecture were genetically closely related to each other (genetic distance: D=0.056) but distantly so toR. ocellatus ocellatus from Saitama Prefecture (D=0.202 or 0.265). Electrophoretic analyses also elucidated the existence of hybrid populations of the two subspecies. The populations ofR. ocellatus kurumeus in Yao City had lower genetic variability and a lower incidence of white coloration on the ventral fins than populations of the same in Saga Prefecture. The present study strongly implies that the introduction of the foreign freshwater fishes with subspecific differentiation, into the original range of indigenous subspecies, should be averted not to bring the genetic pollution.

Key words

Rhodeus ocellatusisozymehybridizationinbreeding effectsgenetic introgression

Copyright information

© The Ichthyological Society of Japan 1996

Authors and Affiliations

  • Yoshikazu Nagata
    • 1
  • Tadashi Tetsukawa
    • 2
  • Takanori Kobayashi
    • 3
  • Ken-ichi Numachi
    • 4
  1. 1.Department of BiologyOsaka Kyoiku UniversityKashiwara, OsakaJapan
  2. 2.Department of Biology, Faculty of EngineeringKansai UniversitySuita, OsakaJapan
  3. 3.Fish Genetics DivisionNational Research Institute of AquacultureMieJapan
  4. 4.Department of Fisheries, Faculty of Marine Science and TechnologyTokai UniversityShimizu, ShizuokaJapan