Applied Microbiology and Biotechnology

, Volume 23, Issue 6, pp 499–501

A rapid method to differentiate between five species of the genusSaccharomyces

  • Johan L. F. Kock
  • Michele Cottrell
  • Pieter M. Lategan
Short contribution

DOI: 10.1007/BF02346068

Cite this article as:
Kock, J.L.F., Cottrell, M. & Lategan, P.M. Appl Microbiol Biotechnol (1986) 23: 499. doi:10.1007/BF02346068

Summary

Long-chain fatty acids of 8 yeast strains representing 5 species of the genusSaccharomyces, were extracted from yeast cells by saponification and analyzed as methyl esters by gasliquid chromatography (GLC). When these species were grown under standard conditions, each produced a distinctive mean fatty-acid “fingerprint” characterized by certain fatty acid compositions. With this method, it was possible to distinguish between the 5 species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguishing between species ofSaccharomyces.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Johan L. F. Kock
    • 1
  • Michele Cottrell
    • 1
  • Pieter M. Lategan
    • 1
  1. 1.Department of MicrobiologyUniversity of the Orange Free StateRepublic of South Africa