Journal of Molecular Evolution

, Volume 43, Issue 5, pp 469–475

Nucleotide sequence and presumed secondary structure of the 28S rRNA of pea aphid implication for diversification of insect rRNA

  • Daisuke Amako
  • O-Yu Kwon
  • Hajime Ishikawa
Articles

DOI: 10.1007/BF02337519

Cite this article as:
Amako, D., Kwon, OY. & Ishikawa, H. J Mol Evol (1996) 43: 469. doi:10.1007/BF02337519

Abstract

Determination of the entire nucleotide sequence of the aphid 28S ribosomal RNA gene (28S rDNA) revealed that it is 4,147 by in length with a G + C content of 60.3%. Based on the nucleotide sequence, we constructed a presumed secondary-structure model of the aphid 28S rRNA which indicated that the aphid 28S rRNA is characterized by the length and high G + C content of its variable regions. The G + C content of the aphid's variable regions was much higher than that of the entire sequence of the 28S rRNA, which formed a striking contrast to those ofDrosophila with the G + C content much lower than the entire 28S molecule. In this respect, the aphid 28S rRNA somewhat resembled those of vertebrates. This is the third report of a complete large-subunit rRNA sequence from an arthropod, and the first 28S rRNA sequence for a nondipterous insect.

Key words

Aphid28S rRNASecondary structure modelG + C contentVariable regionDivergent evolution

Copyright information

© Springer-Verlag New York Inc. 1996

Authors and Affiliations

  • Daisuke Amako
    • 1
  • O-Yu Kwon
    • 1
  • Hajime Ishikawa
    • 1
  1. 1.Department of Biological Sciences, Graduate School of ScienceUniversity of TokyoTokyoJapan
  2. 2.Department of Agricultural BiologyKyungpook National UniversityTaeGuKorea