The extracellular matrix proteins fibulin-1 and fibulin-2 in the early human embryo
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Fibulin-1 and fibulin-2, two recently identified extracellular matrix proteins with a homologous domain structure, are known to bind various extracellular ligands and calcium. In this study, they have been localized at the light microscopical level in human embryos of gestational weeks 4–10, using polyclonal antibodies. Identical localization patterns were observed for the two fibulins in most of the tissues. In the heart, the endocardial cushion tissue and endocardium, but not the myocardium, were stained, as were the basement membrane zones and adventitia of blood vessels. Staining was also observed in the perichondrium and calcifying regions of developing bones. Moreover, reactions occurred with the gut subepithelium and epithelial basement membranes of the skin. Differences in staining patterns, however, were observed in various neural structures. Fibulin-1 was prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. Fibulin-2 was detected primarily within the neuropithelium, spinal ganglia and peripheral nerves. The early embryonic expression of both fibulins indicates specific roles during organ development and, in particular, involvement in the differentiation of heart, skeletal and neuronal structures.
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- The extracellular matrix proteins fibulin-1 and fibulin-2 in the early human embryo
The Histochemical Journal
Volume 28, Issue 2 , pp 109-116
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- 1. Zentrum Anatomie, Abt. Histologie, Universität Göttingen, Kreuzbergring 36, 37075, Göttingen, Germany
- 2. Max-Planck-Institut für Biochemie, 82152, Martinsried, Germany
- 3. Department of Biochemistry and Molecular Biology and Department of Dermatology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania, USA