Antonie van Leeuwenhoek

, Volume 38, Issue 1, pp 369–377

A rapid and specific enrichment procedure forHyphomicrobium spp.

  • Margaret M. Attwood
  • W. Harder

DOI: 10.1007/BF02328108

Cite this article as:
Attwood, M.M. & Harder, W. Antonie van Leeuwenhoek (1972) 38: 369. doi:10.1007/BF02328108


An enrichment procedure for the isolation of stalked bacteria of the genusHyphomicrobium is described. The method is based on the use of an organic C1 compound as carbon and energy source for growth together with anaerobic incubation in the presence of nitrate as an electron acceptor. Optimal conditions for the growth of a number ofHyphomicrobium isolates have been investigated. Applying these conditions,Hyphomicrobium spp. have been enriched from a wide range of natural habitats within 1–2 weeks.

Copyright information

© Swets & Zeitlinger 1972

Authors and Affiliations

  • Margaret M. Attwood
    • 1
  • W. Harder
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHaren (Gr.)the Netherlands
  2. 2.Department of MicrobiologyUniversity of SheffieldEngland