Annals of Surgical Oncology

, Volume 5, Issue 8, pp 699–705

Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

  • Arjen M. Rijken
  • Akiko Umezawa
  • Thomas M. van Gulik
  • Anne Bosma
  • Mirjam M. Polak
  • G. Johan A. Offerhaus
  • Huug Obertop
  • Dirk J. Gouma
Original Articles

DOI: 10.1007/BF02303480

Cite this article as:
Rijken, A.M., Umezawa, A., van Gulik, T.M. et al. Annals of Surgical Oncology (1998) 5: 699. doi:10.1007/BF02303480

Abstract

Background: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC).

Methods: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry.

Results: A significant correlation was found between a low MIB-1 index (<20%) and survival (P<.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found.

Conclusion: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of ⩾20% was associated with decreased survival time.

Key Words

PrognosticationCell proliferationKi-67 antigenNuclear DNA contentBile duct carcinoma

Copyright information

© The Society of Surgical Oncology, Inc. 1998

Authors and Affiliations

  • Arjen M. Rijken
    • 2
    • 1
  • Akiko Umezawa
    • 2
  • Thomas M. van Gulik
    • 2
  • Anne Bosma
    • 1
  • Mirjam M. Polak
    • 1
  • G. Johan A. Offerhaus
    • 1
  • Huug Obertop
    • 2
  • Dirk J. Gouma
    • 2
  1. 1.the Department of Pathology, Academic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Dept. of Surgery, Academic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands