Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma
Received: 16 April 1998 Accepted: 12 July 1998 DOI:
10.1007/BF02303480 Cite this article as: Rijken, A.M., Umezawa, A., van Gulik, T.M. et al. Annals of Surgical Oncology (1998) 5: 699. doi:10.1007/BF02303480 Abstract Background: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). Methods: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. Results: A significant correlation was found between a low MIB-1 index (<20%) and survival ( P<.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. Conclusion: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of ⩾20% was associated with decreased survival time. Key Words Prognostication Cell proliferation Ki-67 antigen Nuclear DNA content Bile duct carcinoma References
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© The Society of Surgical Oncology, Inc. 1998