Chromatographia

, Volume 40, Issue 9, pp 500–506

On-line automated sample preparation-capillary gas chromatography for the analysis of plasma samples

  • A. J. H. Louter
  • R. A. C. A. van der Wagt
  • U. A. Th. Brinkman
Originals

DOI: 10.1007/BF02290259

Cite this article as:
Louter, A.J.H., van der Wagt, R.A.C.A. & Brinkman, U.A.T. Chromatographia (1995) 40: 500. doi:10.1007/BF02290259

Summary

An automated sample preparation module, (the automated sample preparation with extraction columns, ASPEC), was interfaced with a capillary gas chromatograph (GC) by means of an on-column interface. The system was optimised for the determination of the antidepressant trazodone in plasma. The clean-up of untreated plasma was carried out with disposable C18 cartridges, using several washing steps. The analyte was desorbed with methanol, the extract was diluted on-line with buffer and preconcentrated on a PLRP-S trapping column. The trapping column was dried by purging with nitrogen gas. Desorption (phase switching) of the trapping column was carried out with ethyl acetate which was introduced into a retention gap using partially concurrent solvent evaporation conditions. Trazodone was determined by GC with flame ionisation detection (FID). With a 1 ml sample, this resulted in a detection limit for trazodone in plasma of 3 ng/ml. The system showed good linearity and repeatability in the range 0.01–1 μg/ml, thus covering the range of pharmacokinetic/dynamic-to-therapeutic concentrations of trazodone in plasma.

Preliminary results for benzodiazepines are promising. They indicate that the use of a selective detector such as the nitrogen/phosphorus detector, is to be recommended.

Key Words

Gas chromatographyASPECTrazodone in plasmaMethod automation

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1995

Authors and Affiliations

  • A. J. H. Louter
    • 1
  • R. A. C. A. van der Wagt
    • 1
  • U. A. Th. Brinkman
    • 1
  1. 1.Department of Analytical ChemistryFree UniversityAmsterdamThe Netherlands