Comparative media studies in the isolation of Candida albicans from pregnant women Article DOI:
Cite this article as: Smith, A.G., Taubert, H.D. & Towns, C.M. Mycopathologia et Mycologia Applicata (1962) 17: 269. doi:10.1007/BF02279300 Summary
Rice agar and corn meal agar, with and without Tween 80, were evaluated clinically as directly inoculable selective and differential media for the isolation of
C. albicans from vulvovaginal specimens taken from pregnant women. Chlamydospore formation on these media was investigated as a criterion for the identification of C. albicans.
Of 301 patients cultured, 118 (39.2 %) gave positive cultures for yeast-like fungi of the genus
Candida. Of 118 strains for which fermentation patterns were determined, 69 (58.5 %) gave the pattern for C. albicans. Of these, 56 (81.1 %) formed chlamydospores.
Tween 80 was found to exert a very stimulating influence on chlamydospore production. Rice agar with Tween 80 appeared to be the most efficient medium for eliciting chlamydospores. However, since strains of 4 out of 6 species of
Candida isolated were found to sporulate it was concluded that chlamydospore formation is not a reliable criterion for the speciation of C. albicans.
Each of the 4 media served satisfactorily as a directly inoculable selective medium for the isolation of yeast-like fungi of the genus
Candida from vulvovaginal specimens. None of the media appeared to preferentially stimulate chlamydospore production in C. albicans.
Smith is Associate Professor in the Department of Microbiology; Dr. Taubert is a Fellow in the Department of Obstetrics and Gynecology; Mr. Towns is Laboratory Assistant in the Department of Microbiology.
Supported in part by a grant from the Lederle Medical Faculty Awards Committee and in part by United States Public Health Service Grant E-3068.
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