Comparative media studies in the isolation ofCandida albicans from pregnant women
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Rice agar and corn meal agar, with and without Tween 80, were evaluated clinically as directly inoculable selective and differential media for the isolation ofC. albicans from vulvovaginal specimens taken from pregnant women. Chlamydospore formation on these media was investigated as a criterion for the identification ofC. albicans.
Of 301 patients cultured, 118 (39.2 %) gave positive cultures for yeast-like fungi of the genusCandida. Of 118 strains for which fermentation patterns were determined, 69 (58.5 %) gave the pattern forC. albicans. Of these, 56 (81.1 %) formed chlamydospores.
Tween 80 was found to exert a very stimulating influence on chlamydospore production. Rice agar with Tween 80 appeared to be the most efficient medium for eliciting chlamydospores. However, since strains of 4 out of 6 species ofCandida isolated were found to sporulate it was concluded that chlamydospore formation is not a reliable criterion for the speciation ofC. albicans.
Each of the 4 media served satisfactorily as a directly inoculable selective medium for the isolation of yeast-like fungi of the genusCandida from vulvovaginal specimens. None of the media appeared to preferentially stimulate chlamydospore production inC. albicans.
- Bakerspigel, A.: A preferred method for the routine identification of Candida. J. infect. Dis.94: 141–143, 1954.
- Benham, R. W.: Certain monilias parasitic on man. Their identification by morphology and by agglutination. J. infect. Dis.49: 183–215, 1931.
- Benham, R. W.: Species of Candida most frequently isolated from man: methods and criteria for their identification. J. chron. Dis.5: 460–472, 1957.
- Conant, N. F., Smith, D. T., Baker, R. D., Calloway, J. L. &Martin, D. S.: Manual of Clinical Mycology, Ed. 2. Philadelphia: W. B. Saunders Company, 1954.
- Dalmau, L. M.: Remarques sur la technique mycologique. Ann. Parasit.7: 536–545, 1927.
- Dubos, R. J.: Bacterial and Mycotic Infections of Man, Ed. 3. Philadelphia: J. B. Lippincott Company, 1958.
- Fahlberg, W. J., Dukes, C. D. &Guthrie, R. K.: Rapid classification of Candida (Monilia) albicans. J. invest. Dermat.29: 111–118, 1957.
- Gordon, M. A., Bradley, E. G. &Grant, V. Q.: The influence of different types of corn meal agar upon chlamydospore production by Candida albicans. J. Lab. clin. Med.40: 316–320, 1952.
- Kelly, J. P. &Funigiello, F.: Candida albicans: a study of media designed to promote chlamydospore production. J. Lab. clin. Med.53: 807–809, 1959.
- Lewis, G. M., Hopper, M. E., Wilson, J. W. &Plunkett, O. A.: An Introduction to Medical Mycology, Ed. 4. Chicago, The Year Book Publishers, Inc., 1958.
- Martin, D. S., Jones, C. P., Yao, K. F. &Lee, L. E., Jr.: A practical classification of the monilias. J. Bact.34: 99–123, 1937.
- Moss, E. M. &McQuown, A. L.: Atlas of Medical Mycology, Ed. 2. Baltimore, Williams and Wilkins Company, 1960.
- Nickerson, W. J. &Mankowski, Z.: A polysaccharide medium of known composition favoring chlamydospore formation in Candida albicans. J. infect. Dis.92: 20–25, 1953.
- Pollack, J. D. &Benham, R. W.: The chlamydospores of Candida albicans: comparison of three media for their induction. J. Lab. clin. Med.50: 313–317, 1957.
- Reid, J. D., Jones, M. M. &Carter, E. B.: A simple clear medium for demonstration of chlamydospores of Candida albicans. Amer. J. clin. Path.23: 938–941, 1953.
- Rosenthal, L.: Personal communication; cited byTaschdjian (23).
- Seeliger, H.: Ein neues Medium zur Pseudomycelbildung von Candida albicans. Z. Hyg. Infektkr.141: 488–491, 1955.
- Simons, R. D. H. Ph.: Handbook of Tropical Dermatology and Medical Mycology, Vol. 2, Houston, Elsevier Press, Inc., 1954.
- Sina, B. &Reiss, F.: Comparative studies of special media for identifying Candida albicans from other candidas and molds. J. invest. Dermat.29: 263–267, 1957.
- Skinner, C. E. &Fletcher, D. W.: A review of the genus Candida. Bact. Rev.24: 397–416, 1960.
- Smith, D. T. &Conant, N. F.: Zinsser Microbiology, Ed. 12, New York, Appleton, Century, Crofts, Inc., 1960.
- Taschdjian, C. L.: A simply prepared identification medium for Candida albicans. Mycologia,45: 474–475, 1953.
- Taschdjian, C. L.: Routine identification of Candida albicans: current methods and a new medium. Mycologia,49: 332–338, 1957.
- Taubert, H. D. &Smith, A. G.: The clinical use of Taschdjian's medium in the diagnosis of vulvovaginal candidiasis. J. Lab. clin. Med.,55: 820–828, 1960.
- Walker, L. &Huppert, M.: Corn meal-tween agar: an improved medium for the identification of Candida albicans. Amer. J. clin. Path.33: 190–194, 1960.
- Wickerham, L. J.: Taxonomy of yeasts. Tech. Bull. No. 1029, U.S.D.A., 1951.
- Wickerham, L. J. &Rettger, L. F.: A taxonomic study of Monilia albicans with special emphasis on morphology and morphological variation. J. trop. Med.42: 174–177, 1939.
- Comparative media studies in the isolation ofCandida albicans from pregnant women
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