Plant and Soil

, Volume 87, Issue 1, pp 17–29

In vitro production of specialized reproductive torulose hyphae byFrankia strain ORS 021001 isolated fromCasuarina junghuhniana root nodules

  • H. G. Diem
  • Y. R. Dommergues

DOI: 10.1007/BF02277644

Cite this article as:
Diem, H.G. & Dommergues, Y.R. Plant Soil (1985) 87: 17. doi:10.1007/BF02277644


AFrankia strain (ORS 021001) isolated fromCasuarina junghuhniana root nodules was shown to produce four type of structuresin vitro: vegetative hyphae, sporangiospores within sporangia, N2-fixing vesicles, and a fourth type of structure which is described in detail in this report. Structures of this latter type which we propose to call ‘reproductive torulose hyphae: (RTH) result from enlargement and multiple segmentation of vegetative hyphae into torulose chaions of spore-like cells. RTH differ from sporangia in three major aspects: morphology, morphogenesis and outgrowth. RTH play an important role in survival and reproduction ofFrankia strain ORS 021001. Adding activated charcoal to the nutrient medium promotes the formation ofFrankia colonies orginating from RTH.

Key words

Actinorhiza Activated charcoal effect Casuarina endophytes Frankia 

Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • H. G. Diem
    • 1
  • Y. R. Dommergues
    • 1
  1. 1.ORSTOM/CNRSDakarSenegal