Chromatographia

, Volume 35, Issue 5, pp 317–320

Sensitive determination of probenecid in urine samples by reversed-phase liquid chromatography and UV-visible detection using solid-phase extraction techniques for sample clean-up

  • P. Campíns-Falcó
  • R. Herráez-Hernández
  • A. Sevillano-Cabeza
Originals

DOI: 10.1007/BF02277517

Cite this article as:
Campíns-Falcó, P., Herráez-Hernández, R. & Sevillano-Cabeza, A. Chromatographia (1993) 35: 317. doi:10.1007/BF02277517

Summary

This study describes a rapid method for the determination of probenecid in human urine by liquid chromatography with UV detection at 254 nm, after clean-up through a C8 solid-phase extraction column. Liquid chromatography was carried out on a C18-bonded phase using an acetonitrile-acetate buffer (pH=4) gradient elution. Ethacrynic acid was used as internal standard. The system has been applied to the determination of probenecid in the 0.10–100.0 μg/ml concentration range; the limit of detection was 5 ng/mL.

Key Words

Column liquid chromatographyProbenecid in urineSolid-phase extraction

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • P. Campíns-Falcó
    • 1
  • R. Herráez-Hernández
    • 1
  • A. Sevillano-Cabeza
    • 1
  1. 1.Departamento de Química Analítica, Facultad de QuímicaUniversidad de ValenciaBurjassot (Valencia)Spain