Chromatographia

, Volume 38, Issue 1, pp 29–34

Determination of triamterene in urine by HPLC using fluorescence detection and column-switching

  • P. Campíns Falcó
  • R. Herráez-Hernández
  • A. Sevillano-Cabeza
Originals

DOI: 10.1007/BF02275723

Cite this article as:
Campíns Falcó, P., Herráez-Hernández, R. & Sevillano-Cabeza, A. Chromatographia (1994) 38: 29. doi:10.1007/BF02275723

Summary

A liquid chromatographic method incorporating column-switching and fluorimetric detection for the determination of triamterene in untreated urine, is described. The urine samples (5 μL) were directly introduced onto an Hypersil ODS-C18, 30 μm (20 mm×2.1 mm I.D.) pre-column. Polar urinary compounds were removed by flushing the pre-column with water for 1 min, and the analyte was then switched onto an HP-LiChrospher RP C18,5 μm (125 mm×4mm ID) analytical column using an acetonitrile/phosphate buffer gradient elution. Fluorescence detection was performed at 230 nm excitation and 430 nm emission wavelengths. The recovery of drug was 102±2% in the 0.10–20.0 μg/mL concentration range, the limit of detection being 5 ng/mL. A validation of the usefulness of this procedure was accomplished by analysing urine extracts obtained from real samples.

Key Words

Column liquid chromatography Column-switching Determination of triamterene Fluorescence detection 

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • P. Campíns Falcó
    • 1
  • R. Herráez-Hernández
    • 1
  • A. Sevillano-Cabeza
    • 1
  1. 1.Departmento de Química Analítica, Facultad de QuímicaUniversidad de ValenciaBurjassot (Valencia)Spain