, Volume 34, Issue 11, pp 597–600

HPLC analysis of the main oxindole alkaloids fromUncaria tomentosa


  • H. Stuppner
    • Institute of PharmacognosyUniversity of Innsbruck
  • S. Sturm
    • Institute of PharmacognosyUniversity of Innsbruck
  • G. Konwalinka
    • Department of Internal MedicineUniversity of Innsbruck

DOI: 10.1007/BF02269869

Cite this article as:
Stuppner, H., Sturm, S. & Konwalinka, G. Chromatographia (1992) 34: 597. doi:10.1007/BF02269869


The main oxindole alkaloids from the root bark ofUncaria tomentosa were separated by reversed phase HPLC with an acetonitrile/methanol/phosphate buffer solvent gradient. UV detection was carried out at 245 nm. The chromatographic parameters for the separation of the alkaloids were optimized by studying the impact of the pH value of the mobile phase and the column temperature on the separation efficiency of the analytical system. The best separation was achieved with a mobile phase pH of 6.6 and a column temperature of 15°C. The method developed is suitable for the qualitative characterisation and quantitative determination of oxindole alkaloids in crudeUncaria tomentosa extracts and phytopharmaceuticals.

Key Words

Column liquid chromatographyOxindole alkaloidsUncaria tomentosa

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992