Original Paper

Journal of Biomedical Science

, Volume 4, Issue 6, pp 300-307

Infiltrated cells in experimental allergic encephalomyelitis by additional intracerebral injection in myelin-basic-protein-sensitized B6 mice

  • Tzong-Shiann HoAffiliated withDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University
  • , Chia-Ying TsaiAffiliated withDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University
  • , Nina TsaoAffiliated withDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University
  • , Nan-Haw ChowAffiliated withDepartment of Pathology, College of Medicine, National Cheng Kung University
  • , Huan-Yao LeiAffiliated withDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University

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Abstract

We previously reported that murine experimental allergic encephalomyelitis can be induced by an additional intraperitoneal and intracerebral (i.c.) restimulation in resistant B6 mice after standard immunization with myelin antigens in complete Freund's adjuvant andBordetella pertussis coadjuvant. Neutrophils infiltrated into perivascular spaces at 12 h, followed by mononuclear cells 24 h after i.c. injection. In this study, we report that the i.c. injection induced the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). The kinetic expression of ICAM-1 or VCAM-1 on brain endothelial cells paralleled the infiltration of neutrophils and mononuclear cells, respectively. The infiltrated lymphocytes also expressed very late antigen-4 (VLA-4) molecules. The microvascular endothelial cells were positive for VCAM-1, whereas the surrounding mononuclear cells were VLA-4 positive. Furthermore, we found a unique subpopulation of cells with characteristics of CD4-CD8-Vβ8+ markers. The kinetic studies of this population showed that these cells were transiently depleted from 12 to 24 h after i.c. challenge (before the development of clinical symptoms) in cervical lymph nodes. These CD4-CD8-Vβ8+ cells can be expanded by in vitro culture with myelin basic protein or IL-2. No significant changes of CD4+/CD8+ cells were noted. CD4+CD8CD3+ cells were also found in brain by double histochemical stains and were the major infiltrating cells at 24 or 48 h after i.c. challenge.

Key Words

Experimental allergic encephalomyelitis Adhesion molecule CD4CD8CD3+ cells Intracerebral stimulation Cervical lymphatics